Fig. 6: KCNQ1OT1 binds to miR-556-3p to augment CLIC1 expression in GC cells. | Cell Death & Disease

Fig. 6: KCNQ1OT1 binds to miR-556-3p to augment CLIC1 expression in GC cells.

From: Tumor-derived extracellular vesicles shuttle c-Myc to promote gastric cancer growth and metastasis via the KCNQ1OT1/miR-556-3p/CLIC1 axis

Fig. 6: KCNQ1OT1 binds to miR-556-3p to augment CLIC1 expression in GC cells.

A Venn diagram of target genes of miR-556-3p in TargetScan (http://www.targetscan.org/vert_72/) and miRDB (http://www.mirdb.org/), GC-related genes in GeneCards, and upregulated genes. B Prediction of CLIC1 expression in GC and adjacent normal tissues by GEPIA website (Red represented GC, N = 408; Gray represented adjacent normal tissues, N = 211). C, Detection of CLIC1 expression in GC and adjacent normal tissues by RT-qPCR (N = 40). D Targetscan website predicting the binding sites of miR-556-3p and CLIC1. E Dual-luciferase reporter assay was used to verify the targeting relationship between miR-556-3p and CLIC1. F The mRNA expression of CLIC1 detected by RT-qPCR after si-KCNQ1OT1 and miR-556-3p inhibitor treatment. G Western blot was used to detect the protein expression of CLIC1 after si-KCNQ1OT1 and miR-556-3p inhibitor treatment. * represents the comparison between the two groups. *p < 0.05; **p < 0.01; ***p < 0.001. All experiments were repeated 3 times.

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