Fig. 2: Effect of Sidt2 deletion on the total number of lysosomes, the number of acid lysosomes, and the lysosomal environment and acid hydrolase. | Cell Death & Disease

Fig. 2: Effect of Sidt2 deletion on the total number of lysosomes, the number of acid lysosomes, and the lysosomal environment and acid hydrolase.

From: Sidt2 is a key protein in the autophagy-lysosomal degradation pathway and is essential for the maintenance of kidney structure and filtration function

Fig. 2

A Sidt2 protein expression level determination by western blot; B statistical charts of the western blot test results; C Sidt2 mRNA expression level determination by qRT-PCR; D LAMP1 immunofluorescence in MPC5 and SV40 MES 13 cells before and after Sidt2 knockout; E statistical chart of (D); F LysoTracker determination of the number of functional lysosomes before and after Sidt2 knockout in MPC5 and SV40 MES 13 cells; G statistical graph of the LysoTracker results; H Cathepsin B expression in WT and Sidt2−/− mice kidney; I statistical chart of (H); J Cathepsin B expression in MPC5 cells before and after Sidt2 knockout; K statistical chart of (J); L Cathepsin B expression in SV40 MES 13 cells before and after Sidt2 knockout; M statistical chart of (L); N LysoSensor detection of the lysosomal pH change on Sidt2 knockout in MPC5 cells; O LysoSensor detection of the lysosomal pH change on Sidt2 knockout in SV40 MES 13 cells; P electron microscopy application to observe the change in the number of lysosomes in kidney cells on Sidt2 knockout (yellow arrows mark primary lysosomes and green arrows mark secondary lysosomes); Q statistical chart of the (P). *P < 0.05, **P < 0.01, ***P < 0.001.

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