Fig. 3: Expression of CS polymerizing enzymes did not overcome GAG elongation defects in TMEM165-deficient cells. | Cell Death & Disease

Fig. 3: Expression of CS polymerizing enzymes did not overcome GAG elongation defects in TMEM165-deficient cells.

From: TMEM165 a new player in proteoglycan synthesis: loss of TMEM165 impairs elongation of chondroitin- and heparan-sulfate glycosaminoglycan chains of proteoglycans and triggers early chondrocyte differentiation and hypertrophy

Fig. 3

A Fold changes in mRNA expression of CS polymerizing enzymes CHSY1 and CHSY2, and HS polymerizing enzymes EXT1 and EXT2 in tmem165-knockout mouse ATDC5 cells normalized to wild-type mouse ATDC5 cells and B in TMEM165-deficient fibroblasts normalized to fibroblast control cells. qPCR values were normalized for the housekeeping gene ribosomal protein S29 and are expressed as the relative expression compared with control. Data are expressed as mean ± S.D. Statistical analysis was performed with an unpaired Student’s t test (n = 3; *p < 0.05; **p < 0.01). C Detection of decorin in conditioned medium of wild-type and mutant ATDC5 cells transfected with empty vector, Myc-tagged CHSY1, HA-tagged CHSY2 or Myc-tagged CHSY1 and HA-tagged CHSY2 along with decorin expression vector. D Detection of CHSY1 and (E) of CHSY2 in wild-type and tmem165-knockout mouse ATDC5 cells. β-actin was used as loading control (n = 3). Representative images from three independent experiments are shown.

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