Fig. 4: CAFs-secreted IL6 up-regulates LRG1 through activating JAK2/STAT3 signaling. | Cell Death & Disease

Fig. 4: CAFs-secreted IL6 up-regulates LRG1 through activating JAK2/STAT3 signaling.

From: Colorectal cancer-associated fibroblasts promote metastasis by up-regulating LRG1 through stromal IL-6/STAT3 signaling

Fig. 4: CAFs-secreted IL6 up-regulates LRG1 through activating JAK2/STAT3 signaling.

a QRT-PCR analysis of IL-6/IL-8/IL-1β/TGFβ2 mRNA level in CAFs compared to NFs. b & c. QRT-PCR and western blot analysis of LRG1 expression in DLD-1 and HCT-116 treated with vehicle or recombinant IL-6/IL-8/IL-1β/TGFβ2. d, e. DLD1 and HCT-116 were cultured with control medium, CM from CAF1 or treated with neutralizing antibody either targeting IL-6R or IL-6 when cultured with CM from CAFs as indicated. Expression of LRG1 was measured by qRT-PCR and western blot. Blots were probed against LRG1, phosphorylated STAT3(Y705), total STAT3 and β-tubulin. f, g DLD1 and HCT-116 were cultured with control medium, CM from CAFs or treated with HO-3867 (STAT3 inhibitor) or pacritinib (JAK2 inhibitor), when cultured with CM from CAFs as indicated. Expression of LRG1 was subsequently analyzed by qRT-PCR and western blot. Blots were probed against LRG1, phosphorylated STAT3(Y705), total STAT3 and β-tubulin. h, i DLD1 was transfected with control siRNA or two independent siRNA targeting STAT3, followed by culturing with control medium or CM from CAFs. Expression of LRG1 and STAT3 was measured by qRT-PCR and western blot. Blots were probed against LRG1, phosphorylated STAT3(Y705), total STAT3 and β-tubulin. Error bars represent SD; n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001.

Back to article page