Fig. 5: STAT3 regulates LRG1 expression through direct binding to its promoter. | Cell Death & Disease

Fig. 5: STAT3 regulates LRG1 expression through direct binding to its promoter.

From: Colorectal cancer-associated fibroblasts promote metastasis by up-regulating LRG1 through stromal IL-6/STAT3 signaling

Fig. 5: STAT3 regulates LRG1 expression through direct binding to its promoter.

a Schematic illustration of potential STAT3 binding sites located at LRG1 promoter region predicted by JASPAR. b Left: Scheme diagram depicting the design of LRG1 promoter regions in the luciferase reporter plasmids. Right: Activity of different fragments of LRG1 promoter in response to CM from CAF1 was determined by dual-luciferase assay. c Activity of LRG1 promoter with deletion of BS1 or not (named LRG1-BS1 and LRG1-BS1 mutant respectively) was tested by dual-luciferase assay. Graph showing relative activity of either promoter upon co-culture with CM from CAF1 or not. d DLD1 with LRG1 promoter containing BS1 was pre-treated with HO-3867 or pacritinib, followed by incubation with CM from CAF1 or not. Activity of LRG1 promoter was analyzed by dual-luciferase assay. e Top: Scheme depicting the regions where primers were designed for amplifying across LRG1 promoter. Bottom: DLD1 was pre-treated with or without pacritinib, followed by co-culture with CM from CAF1 or not. Quantitative ChIP-PCR assay was performed to detect the occupancy of stat3 protein at the different regions of the LRG1 promoter under indicated conditions. Error bars represent SD; n = 3. **P < 0.01, ***P < 0.001, ****P < 0.0001; ns for no significant.

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