Fig. 2: Ectopic expression of PAARH enhanced HCC cellular malignant phenotype in vitro.

a PAARH expression in SNU-398 and SK-HEP-1 cells with PAARH stable overexpression was measured by qRT-PCR. b Cell growth of SNU-398 and SK-HEP-1 cells with PAARH overexpression was detected using CCK-8 assays. c Cell growth of SNU-398 and SK-HEP-1 cells with PAARH overexpression was detected using EdU incorporation assays. Scale bars, 100 µm. d Cell apoptosis of SNU-398 and SK-HEP-1 cells with PAARH overexpression was detected using caspase-3 activity assays. e Cell migration of SNU-398 and SK-HEP-1 cells with PAARH overexpression was detected using transwell migration assays. Scale bars, 100 µm. f Cell invasion of SNU-398 and SK-HEP-1 cells with PAARH overexpression was detected using transwell invasion assays. Scale bars, 100 µm. Results are shown as mean ± SD based on three independent experiments. **p < 0.01, ***p < 0.001, ****p < 0.0001 by Student’s t test.