Fig. 2: scRNA-seq defines a unique gene expression pattern of calcium-macrophages. | Cell Death & Disease

Fig. 2: scRNA-seq defines a unique gene expression pattern of calcium-macrophages.

From: Danger signal extracellular calcium initiates differentiation of monocytes into SPP1/osteopontin-producing macrophages

Fig. 2: scRNA-seq defines a unique gene expression pattern of calcium-macrophages.

a UMAP of 11 macrophage clusters identified by scRNA-seq analysis. b UMAP of the identified clusters with the detected macrophage population hashtag. c Heatmap of the top 20 DEGs per cluster. Top cluster markers and hashtag macrophage population assignment are provided. d Violin plots representing log-normalized expression values of cluster markers; medians marked by black dots and cluster identity by individual coloring. e Split UMAP and dot plots of relative changes in clusters. f Heatmap of the top 20 DEGs per macrophage population hashtag. a–f scRNA-seq of three tagged macrophage populations (7-day human monocyte-derived macrophages: calcium-macrophages, GM-CSF-macrophages, M-CSF-macrophages) pooled in one sample. g Top 30 genes significantly regulated in day 1 calcium-macrophages compared to monocytes. Calcium-macrophages were differentiated from monocytes of four different donors for the indicated times, and gene expression was analyzed using DNA microarray. Significance levels are marked with * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. h Principal component analysis of monocytes and day 1, day 2, day 4, and day 7 calcium-macrophages.

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