Fig. 4: Identification and validation of EIF5A2 as a HERC3-interaction protein. | Cell Death & Disease

Fig. 4: Identification and validation of EIF5A2 as a HERC3-interaction protein.

From: HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer

Fig. 4

a Exogenously expressed EIF5A2 and HERC3 could interact with each other. HCT116 cells were transfected with indicated plasmids and then were subjected to co-IP. b Endogenous HERC3 could interact with EIF5A2, the lysates of HCT116 cells were subjected to co-IP with anti-HERC3 or anti-EIF5AH2 and control IgG. c The interaction between HERC3 and EIF5A2 depended on the RCC1 domain in HERC3. HCT116 cells were transfected with indicated plasmids and subjected to co-IP. d Detailed hydrogen bonding sites between amino acids in HERC3 and EIF5A2. The structure of proteins was visualized by SWISS-MODEL, and the analysis of the interaction was performed by ClusPro.

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