Fig. 5: Impaired Sertoli cell differentiation and ectopic FOXL2 in XY Nedd4-/- mice.
From: Loss of NEDD4 causes complete XY gonadal sex reversal in mice
Section immunofluorescence for the male sex determinant SRY (green) (A) and Sertoli cell marker SOX9 (green) (B) co-labelled with the germ cell marker DDX4 (magenta) on XY Nedd4-/- embryos and XY wildtype controls between 18ts and 30ts. C Section immunofluorescence for the granulosa cell marker FOXL2 (green) and Sertoli cell marker AMH (magenta) on XY Nedd4-/- embryos alongside XY and XX controls between 21ts and 13.5 dpc. The anterior pole of each gonad is positioned at the top of each panel. Gonads are denoted by a white dotted line. Scale bars = 100μm. RT-qPCR analyses of Sry (D), Sox9 (E) and Foxl2 (F) expression at 11.5 dpc (17–19ts - gonads + mesonephros) and 12.5 dpc (gonads only) on XY Nedd4-/- (KO) (light blue; n = 6), XY controls (Co) (dark blue; n = 6) and XX controls (pink; n = 3). Values are expressed relative to 11.5 dpc XY controls. Mean ± SEM; t-test; n.s. not significant, *p < 0.05, **p < 0.01, ***p < 0.001.
