Fig. 4: DLEU2 competed with SGK1 for interaction with miR-582-5p.

A FISH analysis of subcellular localization of DLEU2 in PC-3 and DU145 cells. B Venn diagrams showing the miRNAs targeting DLEU2 and SGK1. C A schematic drawing showing the possible binding sites of miR-582-5p within DLEU2 and the SGK1 3′-UTR and the corresponding site-specific mutations. D Relative expression of miR-582-5p in PC-3 and DU145 cells stably knockdown DLEU2 using RT-qPCR. E Relative reporter gene activity of vector containing DLEU2 in 293 T cells co-transfected with miR-582-5p mimics. F Correlation between miR-582-5p expression and DLEU2 expression in TCGA database using spearman analysis. G Quantitative analysis of RNA levels of miR-582-5p in prostate cancer cells transfected with miR-582-5p inhibitor or miR-582-5p mimics. H Quantitative analysis of RNA levels of SGK1 in prostate cancer cells transfected with miR-582-5p inhibitor or miR-582-5p mimics. I Quantitative analysis of protein levels of SGK1 in prostate cancer cells transfected with miR-582-5p inhibitor or miR-582-5p mimics. J Relative luciferase reporter assays in 293 T cell lines co-transfected with vector containing the SGK1 3’UTR and miR-582-5p mimics. K Correlation between SGK1 expression and miR-582-5p expression in TCGA database using spearman analysis. L, M Quantitative analysis of SGK1 expression in prostate cancer cells co-transfected with shDLEU2 and miR582-5p inhibitor. Data were indicated as mean ± SD, ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.