Fig. 6: DLEU2 transcription was activated by aberrant expression of E2F2. | Cell Death & Disease

Fig. 6: DLEU2 transcription was activated by aberrant expression of E2F2.

From: E2F transcription factor 2-activated DLEU2 contributes to prostate tumorigenesis by upregulating serum and glucocorticoid-induced protein kinase 1

Fig. 6

A Correlation between E2F2 expression and DLEU2 expression in TCGA database using spearman analysis. B Relative expression of DLEU2 in PC-3 and DU145 cells transfected with E2F2 using RT-qPCR. C Relative luciferase reporter assays in PC-3 and DU145 cells after the co-transfection of plasmid constructs containing the DLEU2 promoter with a E2F2 overexpressing construct. D The DNA motif for E2F2 was obtained from JASPAR. E E2F2 binding sites in DLEU2 promoter were predicted by JASPAR. F Relative luciferase reporter assays in PC-3 cells after the co-transfection of a series of truncated and mutated DLEU2 promoter with a E2F2 overexpressing construct. G ChIP assay demonstrated the direct interactions between E2F2 and DLEU2 promoters in prostate cancer cells. Data were indicated as mean ± SD, ns P ≥ 0.05, *P < 0.05, **P < 0.01, ***P < 0.001.

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