Fig. 2: Overexpression of ZNF32 in CRC cells increased the self-renewal capacity. | Cell Death & Disease

Fig. 2: Overexpression of ZNF32 in CRC cells increased the self-renewal capacity.

From: ZNF32 promotes the self-renewal of colorectal cancer cells by regulating the LEPR-STAT3 signaling pathway

Fig. 2: Overexpression of ZNF32 in CRC cells increased the self-renewal capacity.The alternative text for this image may have been generated using AI.

(A) Western blot analysis of ZNF32, CD133, CD166 and ALDH1 between ZNF32-overexpressing (lv-ZNF32) and control (lv-Vector) SW480 and pCRC1 cells. The image is one represent of three independent experiments. (B) IFA to detect CD133 expression between lv-ZNF32 and lv-Vector in SW480 and pCRC1 cells. The CD133-positive cells were membranous positive (red). The image is one represent of three independent experiments. (micron bar = 50 μm). (C) FCM to confirm CD133 expression between lv-ZNF32 and lv-Vector in SW480 and pCRC1 cells. The image is one represent of three independent experiments. (D) 3D colony-forming assay to analyze the colony formation capacity between lv-ZNF32 and lv-Vector in SW480 and pCRC1 cells. The data presented as the means ± S.Ds. The dots of histogram were used to plot all data. (E) Limiting dilution assay to analyze the number of tumor spheres between lv-ZNF32 and lv-Vector SW480 and pCRC1 cells. The data presented as the means ± S.Ds. The dots of histogram were used to plot all data. (F) CRC cells (lv-ZNF32 and lv-Vector) with different cell numbers (103, 105, 107) were injected subcutaneously, and the tumor formation rate was calculated. There are 5 mice in each group. And each group was repeated 3 times independently. (G) The tumor morphology is shown in the tumor column. And the samples were stained for HE and IHC analysis with Ki-67, CD133 and Tunel. The positive cells were stained brown. The image is one represent of three independent experiments. (micron bar = 20 μm).

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