Fig. 3: RNA-sequence analysis of CRC cells and colorectal CSCs.

(A) Heat map of 2718 differentially expressed genes between bulk cells and colorectal CSCs (SW480 and pCRC1 vs CSCs-SW480 and CSC-pCRC1). Each column represents one sample; each row represents one probe set. Red indicates upregulated expression, while green indicates downregulated expression. The image is one represent of three independent experiments. (B) A volcano plot was arranged using the fold change and P value. The red rectangle represents differentially expressed genes between bulk cells and colorectal CSCs, P < 0.05, fold change ≥ 2.0. The image is one represent of three independent experiments. (C) GO analysis of upregulated genes between bulk cells and colorectal CSCs. The data presented here are from one representative experiment of three independent experiments. (D) GO analysis of downregulated genes between bulk cells and colorectal CSCs. The data presented here are from one representative experiment of three independent experiments. (E) KEGG pathway analysis of upregulated genes between bulk cells and colorectal CSCs. The data presented here are from one representative experiment of three independent experiments. (F) KEGG pathway analysis of downregulated genes between bulk cells and colorectal CSCs. The data presented here are from one representative experiment of three independent experiments.