Fig. 3: Increased MoMFs but decreased restorative Ly6C^low MoMFs in the liver of Manf^Mye-/-mice after APAP treatment.

WT and Manf^Mye-/-mice were injected with APAP. A Representative flow cytometric plots and the statistical quantification of hepatic MoMFs (Ly6G^-CD11b^high F4/80^intermediate). B Representative flow cytometric plots and the statistical quantification of hepatic Ly-6C^hi and Ly-6C^low expressing MoMFs in WT and Manf^Mye-/-mice. C, D MoMFs were isolated from WT mice at 72 h following APAP administration. Manf^Mye-/-mice received (5 × 10⁵ cells) WT-derived MoMFs at the same time as APAP administration, and were sacrificed 48 h later. C Serum ALT levels were measured. D The percentage of necrotic areas in the liver was quantified. Data are presented as mean ± SD. n = 5–7, 3 independent experiments. *P < 0.05, **P < 0.01.