Fig. 4: The rhMANF enhances the restorative macrophage phenotype in vivo and accelerates liver injury resolution. | Cell Death & Disease

Fig. 4: The rhMANF enhances the restorative macrophage phenotype in vivo and accelerates liver injury resolution.

From: Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathway

Fig. 4: The rhMANF enhances the restorative macrophage phenotype in vivo and accelerates liver injury resolution.

WT mice and ManfMye-/-mice were intravenously injected with rhMANF (1.5 mg/kg) at the same time as APAP administration. Mice were sacrificed at 48 h after APAP administration. A The percentages and numbers of hepatic MoMFs, Ly-6Chi expressing MoMFs, and Ly-6Clow expressing MoMFs were quantified by flow cytometric analysis. B Serum ALT activities were determined. C Representative H&E-stained liver sections. n = 5–7 sections/mouse. D Necrotic areas in the liver were quantified by ImageJ software. Data are presented as mean ± SD, n = 5–7 mice per group, 3 independent experiments.

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