Fig. 2: NK cells reduce senescent markers and decrease the SASP phenotype in human adipose tissue. | Cell Death & Disease

Fig. 2: NK cells reduce senescent markers and decrease the SASP phenotype in human adipose tissue.

From: Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice

Fig. 2

Flow cytometry to detect expression of (A) perforin and (B) CD69 was performed after 48-h co-incubation with NK cells and human adipose tissue, treated with conditional medium from control (CON) or senescent (SEN) preadipocytes for 24 h. Cells were gated in CD3-CD56+ location. n = 3. Data are presented as means ± SD. Differences were assessed by the two-tailed unpaired nonparametric t-test. *P < 0.05, **P < 0.01. C Senescent markers for P16 and P21 were detected by Western blotting. n = 3. D Secreted proinflammatory cytokines in conditional medium from adipose tissue was collected 48-h after removing the NK cells. n = 3. Data are presented as means ± SD. Differences were assessed by the two-way ANOVA test. ns P > 0.05, *P < 0.05, **P < 0.01. Two parallel samples were set in each experiment and three independent experiments were performed for each result.

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