Fig. 1: Altered gene expression profile in L02-HBx and L02-vector cells. | Cell Death & Disease

Fig. 1: Altered gene expression profile in L02-HBx and L02-vector cells.

From: HBx increases chromatin accessibility and ETV4 expression to regulate dishevelled-2 and promote HCC progression

Fig. 1

A Heatmap of differentially expressed genes (DEGs) induced by HBx transfection in the RNA-seq analysis. B Gene set enrichment analysis (GSEA) comparing the gene sets of DNA binding TF activity in the HBx group. NES represents the normalized enrichment score (P < 0.01). C Twenty-two genes were identified after intersection analysis between the DNA binding TF activity signaling pathway and 568 driver genes using Venn diagram software. D Kaplan–Meier survival curve analysis representing probabilities of overall survival (OS) in 371 HCC patients according to ETV4, ETV5, MLLT1, and IKZF3 expression. Statistical analysis was conducted using Student’s t-test and the log-rank test. E Four of 22 intersectional genes expression showed significant differences in 371 HCC tissues and 50 nontumor tissues from TCGA database. F The mRNA level of ETV4 was detected by qRT–PCR in 20 pairs of HCC tissues. G The statistical graph shows the mRNA expression levels of ETV4 in 20 pairs of HCC tissues. H ETV4 protein level was detected by Western blotting (WB) in ten pairs of HCC tissues.

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