Fig. 6: ETV4 upregulates DVL2 expression and its transcriptional activity by directly binding to the DVL2 promoter. | Cell Death & Disease

Fig. 6: ETV4 upregulates DVL2 expression and its transcriptional activity by directly binding to the DVL2 promoter.

From: HBx increases chromatin accessibility and ETV4 expression to regulate dishevelled-2 and promote HCC progression

Fig. 6

A A schematic representation of the ChIP-PCR and ChIP-qPCR primers designed around the DVL2 promoter region. Seven predicted binding sites were designed to be included in five pairs of primers. B ChIP-PCR assay with ETV4 antibody to validate the direct interaction between ETV4 and the DVL2 promoter region in Hep3B and HepG2.2.15 cells. The results emphasize that ETV4 can bind at site 1 (−918 bp to −909 bp) of the DVL2 promoter region. Cell lysates before precipitation were removed separately as input (1%). IgG was a negative control provided by the kit. H3 was a positive control provided by the kit. C ChIP-qPCR assay with ETV4 antibody to validate the direct interaction between ETV4 and the DVL2 promoter region in Hep3B and HepG2.2.15 cells. The results confirm that ETV4 can bind at site 1 (−918 bp to −909 bp) of the DVL2 promoter region. IgG was a negative control provided by the kit. D Schematic diagram of the construction of a dual luciferase reporter gene construct. The DVL2 promoter fragment was inserted into the pGL3-Basic plasmid. ProFL represents the full length of the DVL2 promoter region, Pro1 represents −1000 bp to −900 bp, Pro2 represents −1000 bp to −950 bp, and mutPro1 indicates that Pro1 has been mutated. E The double luciferase reporter gene experiment showed that ETV4 combined with DVL2 promoter site 1. When predicted binding site 1 was mutated, the luciferase activity was rescued.

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