Fig. 1: ARDS-developing mice show more abundant apoptotic cells in lungs and bronchoalveolar lavage than HP-developing mice.
From: Blockade of caspase cascade overcomes malaria-associated acute respiratory distress syndrome in mice
Lung tissues and bronchoalveolar lavage (BAL) from ARDS- and HP-developing mice were collected from Plasmodium berghei-DBA/2 mice infected mice, on the 7th dpi. A, B Distribution of apoptotic [fluorescent] cells, in the lungs. B Quantification of apoptotic cells by pulmonary tissue area (µm2). C–E Apoptotic cells [Annexin V+] measured in BAL and necrotic cells stained by 7AAD dye. C Representative flow cytometry dot plots from in vivo cell staining (Annexin V− and 7AAD−), apoptotic cells (Annexin V+), and late apoptotic cells (Annexin V+ and 7AAD+), performed by flow cytometry. D Frequency and E number of Annexin V+ in BAL. Graphs represent two grouped experiments expressed with mean ± SE, by Mann–Whitney (n = 7–9 per group; **p < 0.05; **p < 0.01; ***p < 0.001). Representative image F of apoptosis in endothelial cells (arrow) and leukocytes (arrowhead), stained by TUNEL (magnification: ×630, scale bar: 10 μm). Dashed lines: average of NI. 7AAD: 7-Amino-Actinomycin D; BAL: bronchoalveolar lavage; NI: non-infected mice; ARDS: acute respiratory distress syndrome; HP: hyperparasitemia.
