Fig. 4: LG3 is located within large autolysosomes.
From: Autolysosomes and caspase-3 control the biogenesis and release of immunogenic apoptotic exosomes
A LG3 immunogold labeling in HUVECs serum-starved (SS) for 2 h or 3 h showing the presence of LG3 in an autophagosome (A, arrow), fused compartments (arrow), and large autolysosomes (AL, arrow). B Electron micrograph of HUVECs serum starved (SS) for 3 h showing LG3 immunogold labeling in a large autolysosome (AL) releasing its content in the extracellular compartment (representative of one experiment). C Confocal microscopy of HUVECs exposed to normal (N) or SS for 3 h (blue, DAPI; green, perlecan/LG3; and red, LAMP2) (representative of three independent experiments). D Representative 3D reconstruction of LAMP2+ vacuoles with transparent or opaque surfaces using Imaris software of HUVECs exposed to normal (N) or SS for 3 h (green, perlecan/LG3; and red, LAMP2). Scale bar = 500 nm unless specified otherwise.
