Fig. 8: Effect of 4-OI on the Nrf2 pathway in LPS-treated microglia. | Cell Death & Disease

Fig. 8: Effect of 4-OI on the Nrf2 pathway in LPS-treated microglia.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 8

A, B The translocation of Nrf2 into the nucleus and cytoplasm of HO-1 and NQO1 expression were detected by western blot and quantified by Image J software. C, D The Nrf2 was detected by immunofluorescence combined with DAPI staining for nuclei (scale bar: 50 μm). E, F After treatment with 4-OI, Nrf2 in the nucleus and HO-1 and NQO1 in the cytoplasm were detected with western blot. G, H The protein expression of iNOS, COX2, IL-6 were detected by western blot. The data are presented as the mean ± SD. n = 5. NS not significant, *P < 0.05, **P < 0.01.

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