Fig. 9: 4-OI reduces inflammation after spinal cord injury in mice and accelerates functional recovery. | Cell Death & Disease

Fig. 9: 4-OI reduces inflammation after spinal cord injury in mice and accelerates functional recovery.

From: Immune-responsive gene 1/itaconate activates nuclear factor erythroid 2-related factor 2 in microglia to protect against spinal cord injury in mice

Fig. 9

A, B Basso mouse scale (BMS) and inclined plane test for the indicated groups at days 1, 3, 7, 14, 21, and 28 (n = 8–12). #P < 0.05, ##P < 0.01 relative to the SCI group. C Photos of mice footprints at day 28 after SCI. D, E Quantification of stride width and toe dragging in the footprint analysis. F The transected spinal cord sections from each group were analyzed at day 28 by HE staining and Nissl staining. The black arrows indicate motor neurons (scale bar: 100 μm and 50 μm). G Quantitative analysis of Nissl positive motor neurons in the anterior horn of spinal cord in each group. H, I The expression of iNOS and COX2 in the spinal cord tissues of each group were detected at day 28 by western blot and quantified by Image J software. J–M The iNOS/NeuN, Cleave-caspase3/NeuN and Nrf2/NeuN in spinal cord injury were detected at day 28 by immunofluorescence combined with DAPI staining for nuclei (scale bar: 25 μm). The data are presented as the mean ± SD. n = 6 (except for BMS and inclined plane test). *P < 0.05, **P < 0.01.

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