Fig. 2: Ubc9 deficiency manifests distinctive effect on peripheral T-cell subsets.
From: SUMOylation of PDPK1 Is required to maintain glycolysis-dependent CD4 T-cell homeostasis
A Shown is the CD44+ CD62Llo activated effector T cells (n = 3 mice per group) in peripheral CD4 T cells (WT: 19.80 ± 1.27% vs. KO: 78.93 ± 1.63%, p < 0.001). B, C Effector T-cell subsets, including Th1 (WT: 2.19 ± 0.20% vs. KO: 5.86 ± 0.83%, p < 0.05), Th17 (WT: 0.77 ± 0.04% vs. KO: 5.20 ± 0.37%, p < 0.001), and Th2 (WT: 0.42 ± 0.01% vs. KO: 0.51 ± 0.01%, p < 0.01) were determined by flow cytometry (n = 3). D Treg cell percentage (WT: 7.17 ± 0.65% vs. KO: 4.57 ± 0.56%, p < 0.05) was examined in peripheral CD4 T cells (n = 4). E–H CD4 naive T cells were labeled with CFSE, and then differentiated under Th1, Th2, Th17, and Treg conditions for 3–5 days. Th1: WT: 25.75 ± 0.25% vs. KO: 25.65 ± 0.65%, p = 0.90; Th2: WT: 11.50 ± 0.70% vs. KO: 11.35 ± 1.35%, p = 0.93; Th17: WT: 30.35 ± 0.25% vs. KO: 31.00 ± 1.90%, p = 0.77; and Treg: WT: 86.55 ± 1.05% vs. KO: 86.90 ± 1.00%, p = 0.83. Differentiation efficiency and proliferation were shown in the bar graph and flow histograms (2-times biological replication for Th2 and Th17; 3-times biological replication for Th1 and Treg). The p-value was determined by Student’s unpaired t-test.
