Fig. 5: RAB3B could promote the DDP resistance of HSCC by inducing autophagy. | Cell Death & Disease

Fig. 5: RAB3B could promote the DDP resistance of HSCC by inducing autophagy.

From: Encoding gene RAB3B exists in linear chromosomal and circular extrachromosomal DNA and contributes to cisplatin resistance of hypopharyngeal squamous cell carcinoma via inducing autophagy

Fig. 5

A Relative mRNA expression of RAB3B in FaDu/DDP cells and FaDu cells was determined by qRT-PCR. B Expression of RAB3B in FaDu/DDP cells and FaDu cells was determined by Western blot. C Sensitivities of FaDu/DDP cells and FaDu cells under different concentrations of DDP were determined by CCK-8 assay. D Sensitivities of FaDu/DDP cells infected with RAB3B shRNA or not under different concentrations of DDP were determined by CCK-8 assay. E Sensitivities of FaDu cells transfected with RAB3B overexpression plasmid or not under different concentrations of DDP were determined by CCK-8 assay. F Protein levels of LC3B and p62 in FaDu/DDP cells and FaDu cells were determined by Western blot. G Protein levels of LC3B and p62 in FaDu/DDP cells infected with RAB3B shRNA or not were determined by Western blot, and the protein levels of LC3B and p62 in FaDu cells transfected with RAB3B overexpression plasmid or not were determined by Western blot. H Autophagy was evaluated in FaDu/DDP cells infected with RAB3B shRNA or not using TEM. I FaDu/DDP cells stably expressing stubRFP-sensGFP-LC3 under shRNA transfection were observed by fluorescence microscopy. J Sensitivities of FaDu cells transfected with RAB3B overexpression plasmid, cotransfected with RAB3B overexpression plasmid and 3MA or not under different concentrations of DDP were determined by CCK-8 assay. K Protein levels of LC3B and p62 in FaDu cells transfected with RAB3B overexpression plasmid, cotransfected with RAB3B overexpression plasmid and 3-MA or not were determined by Western blot. L Effects of sh-RAB3B and/or DDP on the viability of PDO (scale bar, 100 μm). M Measurement of the growth of PDO in response to knockdown of RAB3B and DDP. Data are the means ± SD (n = 3 independent experiments); *p < 0.05, **p < 0.01, ***p < 0.001, #p > 0.05.

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