Table 1 The measurement of ferroptosis.

From: Targeting ferroptosis in acute kidney injury

Detection

Methods

References

Cell viability, cytotoxicity, and death

CCK-8, LDH release, and TUNEL

[136, 137]

Iron levels

Flow cytometry or confocal via Phen Green SK probes, chromogen method, Iron/TIBC reagent

[138]

ROS levels and lipid peroxidation

MDA, 4HNE, flow cytometry via BODIPY and DCFH-DA, JC-1, GSH

[139,140,141,142]

Mitochondrial oxidative stress

Mito TEMPO

[143]

Biomarker proteins

NRF2, NCOA4, HO-1, GPX4, FTH, COX2, PTGS2, ACSL4, NOX1, SLC7A11, metallothionein-1

[126]

Morphology

TEM

[144, 145]

  1. Note: CCK-8 Cell Counting Kit, NRF2 nuclear respiratory factor 2, NCOA4 nuclear receptor coactivator 4, HO-1 heme oxygenase-1, FTH heavy peptide ferritin, TEM transmission electron microscopy, ACSL4 acyl-CoA synthetase long-chain family member 4, NOX1 NADPH oxidase activator 1, SLC7A11 cysteine/glutamate antiporter solute carrier family 7 member 11.
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