Fig. 2: NEDD4L expression in MM.
From: NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma
A Western blotting and qPCR assays of NEDD4L expression in human MM cell lines and PBMCs. B Immunofluorescence staining analysis of the subcellular localization of NEDD4L in CD138+ cells from BM biopsy tissues of MM patients. Nuclei were stained with DAPI. Scale bars, 50 μm. C Western blotting and qPCR analyses of NEDD4L expression in human MM cell lines treated with different concentrations of Bor (0, 5, 10, and 20 nM) for 24 h. D Western blotting analysis of the subcellular localization of NEDD4L in HMCLs treated with or without Bor (10 nM). Cyto refers to the cytoplasm, and Nuc refers to the nucleus. E ARP-1 and RPMI8226 cells were treated with or without Bor (10 nM), NQDI-1 (15 μM), Baxi (15 μM), or Z-VAD-FMK (40 μM). Whole-cell extracts were analyzed by western blotting with anti-NEDD4L and anti-GAPDH antibodies. NQDI-1: an ASK1 inhibitor, Baxi_ a Bax-mediated apoptosis inhibitor.
