Fig. 3: NEDD4L regulates the Bor sensitivity in MM cells.
From: NEDD4L binds the proteasome and promotes autophagy and bortezomib sensitivity in multiple myeloma
A Western blotting and qPCR analyses showing the expression of NEDD4L in NEDD4L-KD and control cells. “ns” means “nonsignificant”. B CCK-8 cell proliferation assay in cell lines with stable NEDD4L KD with or without exposure to 3 nM Bor or 1% FBS for five days. C, D Cell apoptosis was detected by CCK-8 and flow cytometry in cell lines with stable NEDD4L KD treated with the indicated concentration of Bor for 24 h. E Western blotTING analysis showing the expression of NEDD4L in NEDD4L-overexpressing and control cells. Cell apoptosis was detected by CCK-8 and flow cytometry in cell lines with stable NEDD4L overexpression treated with the indicated concentration of Bor for 24 h. F Cell cycle assay performed by flow cytometry showing the percentages of cells in G1, S, and G2 phase in NEDD4L-KD cells treated with or without Bor for 24 h. The histograms show the percentages of HMCLs in G1, S, and G2 phase in three independent experiments. G Western blot assay of NEDD4L and P21 in cells with stable NEDD4L KD after treatment with 10 nM Bor for 24 h.
