Fig. 6: TPX2 promotes the phosphorylation of P65 and activates the NF-κB signaling pathway.
From: Downregulation of TPX2 impairs the antitumor activity of CD8+ T cells in hepatocellular carcinoma
A Heatmap of the differentially expressed genes induced by TPX2 upregulation (LV-TPX2) in human tumor-infiltrating CD8 + T cells in HCC. B KEGG pathway enrichment of the differentially expressed genes induced by TPX2 upregulation. The enriched pathways with Q-value < 0.05 (Q-value < 0.05 was considered significant) was shown here. C The expression levels of TPX2, NF-κB, and p-P65 in LV-TPX2- or LV-shRNA-transfected CD8 + T cells isolated from HCC TILs or PBMCs. D p-P65 staining in Lv-Tpx2- or Lv-shRNA-transfected CD8 + T cells. Scale bar: 50 μm. DAPI 4′,6-diamidino-2-phenylindole, for nuclear staining, LV-NC lentivirus used as a control, LV-shRNA lentivirus used to knock down the human TPX2 gene, Lv-shRNA lentivirus used to knock down the mouse Tpx2 gene, Lv-Tpx2 lentivirus used to overexpress the mouse Tpx2 gene, LV-TPX2 lentivirus used to overexpress the human TPX2 gene, PBMCs peripheral blood mononuclear cells, p-P65 phosphorylated P65, TIL tumor-infiltrating lymphocyte. Raw western blot results can be found in the Original data file.
