Fig. 1: Ionizing radiation-induced lncRNA CRYBG3 regulates the stiffness of the tumor.

A Representative atomic force microscopy stiffness-map images (left) and quantifications (right) of stiffness in xenografted tumors exposed to 10-Gy X-rays (IR group, n = 7) or control (control group, n = 7). B Representative immunofluorescence images of p-MLC2 (green) and phalloidin (red) in xenografted tumors exposed to 10-Gy X-rays or control. The samples were frozen, sectioned, and stained. Scale bars, 30 μm. C Representative stiffness-map images (left) and quantifications (right) of stiffness in xenografted tumors treated with lncRNA control (LNC Control, n = 6) or lncRNA CRYBG3- (LNC CRYBG3, n = 6) overexpressed adenovirus. D Representative immunofluorescence images of p-MLC2 (green) and phalloidin (red) on indicated xenografted tumors. Scale bars, 30 μm. E Representative stiffness-map images (left) and quantifications (right) of xenografted tumors subcutaneously injected by shRNA against lncRNA CRYBG3 (n = 6) or control shRNA (n = 6). F Representative immunofluorescence images of p-MLC2 (green) and phalloidin (red) on indicated xenografted tumors. Scale bars, 30 μm. G Representative bright-field and stiffness-map images in A549 cell-overexpressed lncRNA control or lncRNA CRYBG3. The right panels were the quantification of average Young’s modulus. H Representative immunofluorescence images of p-MLC2 (green) and phalloidin (red) in A549 cells. Scale bars, 20 μm. Data are presented as mean ± s.d. P-values were calculated by unpaired Student’s t-test. **p < 0.01; ****p < 0.0001.