Fig. 1: Exposure to submicromolar CuET induces proteotoxic stress, protein aggregation, and activation of UPR and heat-shock pathways in MM cell lines. | Cell Death & Disease

Fig. 1: Exposure to submicromolar CuET induces proteotoxic stress, protein aggregation, and activation of UPR and heat-shock pathways in MM cell lines.

From: A drug repurposing strategy for overcoming human multiple myeloma resistance to standard-of-care treatment

Fig. 1

A CuET induces poly-ubiquitinated proteins (K-48-Ub), increases XBP1s and ATF4 proteins (markers of unfolded protein response, UPR), and accumulates heat shock protein HSP70 in AMO1 and MM1.S myeloma cell lines. B Immobilization of NPL4, HSP70, and K48-Ub- proteins in Triton X-100 pre-extracted pellet fraction of CuET–treated (500 nM, 5 h) AMO1 and MM1.S cells. C Immunofluorescence analysis of CuET (500 nM) and BTZ (10 nM) induced K48- poly-Ub proteins together with HSP70 in MM cell lines without/with pre-extraction step (5 h treatment). Scale 10 μM.

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