Fig. 2: The extent of AP-related overactivated autophagy was positively regulated by the incubation level of ATG7. | Cell Death & Disease

Fig. 2: The extent of AP-related overactivated autophagy was positively regulated by the incubation level of ATG7.

From: ATG7-enhanced impaired autophagy exacerbates acute pancreatitis by promoting regulated necrosis via the miR-30b-5p/CAMKII pathway

Fig. 2

A Representative TEM photos of pancreatic tissues harvested from the rats that were subjected to sham operation, AP, AP + Lv-ATG7 and AP + Lv-sh-ATG7 for 6 h since AP induction. The percentage of autophagic vacuoles (white arrows) per cytoplasm area was calculated. Bar = 5 μm. B Representative Western blot images and quantifications of ATG7, p62, LAMP-2 protein expression and LC3 conversion in pancreatic tissues as described above. β-actin was used as the protein loading control. C Representative fluorescent photographs of autophagy flux assay in mRFP-GFP-LC3 tagged AR42J cells that were subjected to control, AP, AP + Lv-ATG7 and Lv-sh-ATG7 for 3 h since AP induction. The number of autophagic dots and ratio of red dots (autolysosomes) to yellow dots (autophagosomes) per cell was calculated. Bar = 50 μm. Data were presented as mean ± SD (N ≥ 3). ∗P < 0.05 versus sham or control, ^P < 0.05 versus AP, and *P < 0.05 versus AP + Lv−ATG7. AP acute pancreatitis, CAMKII calcium/calmodulin-dependent protein kinase II, LAMP-2 lysosome-associated membrane protein-2, LC3 microtubule-associated protein 1 light chain 3, SD standard deviation, TEM transmission electron microscopy.

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