Fig. 4: ATG7 overexpression promoted the activation of CAMKII via miR-30b-5p inhibition. | Cell Death & Disease

Fig. 4: ATG7 overexpression promoted the activation of CAMKII via miR-30b-5p inhibition.

From: ATG7-enhanced impaired autophagy exacerbates acute pancreatitis by promoting regulated necrosis via the miR-30b-5p/CAMKII pathway

Fig. 4

A An online bioinformatic analysis and a microarray analysis of miRNA using five pairs of pancreatic tissues of rats with or without AP modeling were performed to predict the potential upstream miR targeting CAMKII mRNA. B Relative luciferase activities of the reporters harboring the wild-type (WT) or the mutated (MUT) CAMKII 3′-untranslated region (UTR) that were subjected to miR-30b-5p mimic or negative control. C Relative miR-30b-5p expressions in cells that were subjected to control, AP, AP + Lv-ATG7 and Lv-sh-ATG7 for 3 h since AP induction. Data were presented as mean ± SD (N ≥ 3). ∗P < 0.05 versus control, ^P < 0.05 versus AP, and *P < 0.05 versus AP + Lv-ATG7. AP acute pancreatitis, CAMKII calcium/calmodulin-dependent protein kinase II, SD standard deviation.

Back to article page