Fig. 6: Fbxo45 negatively regulates USP49-mediated cell proliferation, migration, and invasion. | Cell Death & Disease

Fig. 6: Fbxo45 negatively regulates USP49-mediated cell proliferation, migration, and invasion.

From: Fbxo45 facilitates pancreatic carcinoma progression by targeting USP49 for ubiquitination and degradation

Fig. 6

A IB analysis of WCLs derived from Panc-1 cells transfected with the indicated plasmids. B MTT assays to detect the proliferation of Panc-1 cells transfected with the indicated siRNAs and cDNA plasmid. Data are shown as the mean ± SD of three independent experiments. ***p < 0.001 compared to control, ###p < 0.001 compared to Fbxo45 siRNA-2 alone or USP49 siRNA-2 alone. C Terminal deoxynucleotidyl transferase dUTP nick end labeling (Tunel) assays to detect cell apoptosis of Panc-1 cells transfected with the indicated siRNAs. ***p < 0.001 compared to the control, ##p < 0.01 compared to Fbxo45 siRNA-2 alone or USP49 siRNA-2 alone. D Wound healing assays to analyze the cell migratory capacity of Panc-1 cells transfected with the indicated siRNAs. ***p < 0.001 compared to control, ##p < 0.01 compared to Fbxo45 siRNA-2 alone or USP49 siRNA-2 alone. E Transwell assays to analyze the cell migration and invasion capacity of Panc-1 cells transfected with the indicated siRNAs. **p < 0.01, ***p < 0.001 compared to control, ###p < 0.001 compared to Fbxo45 siRNA-2 alone or USP49 siRNA-2 alone. F Transwell assays to analyze the migration and invasion capacity of Panc-1 cells transfected with the indicated plasmids. **p < 0.01 compared to indicated group.

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