Fig. 2: Tumor cells in diversity-high group possess stemness characteristics.
From: Single cell transcriptional diversity and intercellular crosstalk of human liver cancer
A Heatmap of upregulated genes of tumor cells in the diversity-high groups compared to diversity-low groups. B Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of upregulated genes of diversity-high groups in Fig. 2A. C Heatmap of genes in diversity-high and diversity-low groups belong to stem cell differentiation and stem cell maintenance gene sets. D Violin plot of stem cell differentiation scores for the clusters in Fig. 2A. E t-SNE plots showing the expression levels of ICAM1, SOX4, ALDH1A1 and BMI1 from the diversity-high and diversity-low groups of tumor cells. F Staining of SOX4 and BMI1 in tumor tissue from liver cancer patients. Bar, 100μm. At least two independent experiments were performed for all data. For curve Figures and bar Figures, data are presented as means ± SD. Unpaired Student’s t-tests were used for comparing two variables. One-way ANOVA was used for multiple variables comparison.
