Fig. 7: Collagen-mediated the target genes of YAP facilitated the stemness of tumor cells.
From: Single cell transcriptional diversity and intercellular crosstalk of human liver cancer
A YAP and IgG peaks are ranked from the strongest to weakest signal. B Integrative analysis combining the data from ChIP-seq with the 1796 genes screened in Fig. 4A. C KEGG analysis performed with the 164 genes identified in (B). D Motif analysis of YAP ChIP-seq. E ChIP experiment performed with YAP antibody in Huh7 cells treated with different concentrations of collagen for 3 days. F mRNA levels of MYC, SOX9, SMAD2, and OLIG2 in Huh7 cells treated with different concentrations of collagen for 3 days. G t-SNE plots showing the expression levels of SOX9 and SMAD2 in tumor cells. H Nuclear colocalizations of YAP and SOX9 in sphere formation assays of Huh7 cells. Bars, 100 µm. I Schematic outline showing that TAF Involves in Transcriptional Diversity via Activating YAP Signaling in Liver cancer. At least two independent experiments were performed for all data. Quantified data are presented as the means ±SD. Unpaired Student’s t tests were used for comparing two variables and one-way ANOVA was used for comparing multiple variables. At least two independent experiments were performed for all data. *P < 0.05; **P < 0.01; ***P < 0.001; n.s, no significance in comparison with the control group.
