Fig. 6: TFF3 activated WNT signaling by inducing WNT ligand expression in PDAC. | Cell Death & Disease

Fig. 6: TFF3 activated WNT signaling by inducing WNT ligand expression in PDAC.

From: Trefoil factor 3 promotes pancreatic carcinoma progression via WNT pathway activation mediated by enhanced WNT ligand expression

Fig. 6: TFF3 activated WNT signaling by inducing WNT ligand expression in PDAC.

A Western blot analysis was performed to determine the expression of WNT-related proteins in Capan-1-TFF3, SW1990-shTFF3, and the corresponding control cells. β-actin was used as input control. B Representative micrographs of IHC staining for CTNNB1 in the indicated xenografts. Scale bar, 20 μm. C Relative TOP/FOP luciferase activity analysis was performed to determine the change of CTNNB1/TCF transcription activity in Capan-1-TFF3, SW1990-shTFF3, and the respective control cells. D Relative TOP/FOP luciferase activity analysis was performed to determine the change of CTNNB1/TCF transcription activity in Capan-1 and SW1990 cells after AMPC treatment. E Western blot analysis was performed to determine the protein expression of stemness-associated genes in Capan-1-TFF3, SW1990-shTFF3, and the corresponding control cells. β-actin was used as input control. F Spheroid formation assay for Capan-1-TFF3, SW1990-shTFF3, and the respective control cells. G, H TOP/FOP luciferase activity analysis was performed to determine the change of CTNNB1/TCF transcription activity in Capan-1-TFF3 and the control cells after the suppression of WNT ligand secretion with 10 μM IWP-2 (G) or LGK-974 (H) treatment for 48 hours.

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