Fig. 4: OTUD3 de-polyubiquitylated IRP2.
From: Deubiquitylase OTUD3 prevents Parkinson’s disease through stabilizing iron regulatory protein 2
a, b HEK293T cells transfected with the indicated constructs or siRNA were treated with MG132 for 8 h before collection with 100 µg/ml FAC or not. c IRP2 ubiquitylation was analyzed in OTUD3−/− MEFs. d The IRP2 ubiquitylation linkage was analyzed in HEK293T cells transfected with IRP2, OTUD3, and the indicated ubiquitin Lys 0, Lys 48-only, or Lys 63-onlyplasmids. All panels are representative results of three or more independent experiments. The uncropped western blots can be found in Supplemental Material.
