Fig. 4: LIN00963 enhances the tumorigenicity of ECSCs. | Cell Death & Disease

Fig. 4: LIN00963 enhances the tumorigenicity of ECSCs.

From: UPF1 contributes to the maintenance of endometrial cancer stem cell phenotype by stabilizing LINC00963

Fig. 4: LIN00963 enhances the tumorigenicity of ECSCs.The alternative text for this image may have been generated using AI.

A LIN00963 expression levels in EC tissues (n = 58) compared to normal tissues (n = 32) detected using qRT-PCR. Data are presented as the means ± SEM, **P < 0.01. B Effects of LIN00963 overexpression or knockdown on SOX2, OCT4, and NANOG expression assessed using western blotting. The results are presented as the ratio of the integrated density values of SOX2, OCT4, and NANOG versus Tubulin. The graphs represent the alteration in the oe-LIN00963 group and the sh-LIN00963 group relative to their respective control groups (protein of interest/Tubulin equal to 1). C Effects of LIN00963 on self-renewal capacity assessed using serial sphere formation assay. D Effects of LIN00963 on carboplatin resistance assessed using the sphere formation assay. E Effects of LIN00963 on carboplatin resistance assessed using the CCK-8 assay. F Effects of LIN00963 on proliferation assessed using the CCK-8 assay. G Effects of LIN00963 on migration and invasion assessed using the Transwell assay. H Effects of LIN00963 on apoptosis assessed using flow cytometry analysis. I Effects of LIN00963 on cell cycle progression assessed using flow cytometry analysis. Data are presented as the means ± SEM (n = 3, each group), *P < 0.05, **P < 0.01, ***P < 0.001 vs. oe-NC group, #P < 0.05, ##P < 0.01, ###P < 0.001 vs. sh-NC group. Scale bars, 50 μm.

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