Fig. 5: UPF1 regulates the biological behavior of ECSCs by stabilizing LINC00963. | Cell Death & Disease

Fig. 5: UPF1 regulates the biological behavior of ECSCs by stabilizing LINC00963.

From: UPF1 contributes to the maintenance of endometrial cancer stem cell phenotype by stabilizing LINC00963

Fig. 5: UPF1 regulates the biological behavior of ECSCs by stabilizing LINC00963.The alternative text for this image may have been generated using AI.

A LIN00963 RNA half-life measured using qRT-PCR after actinomycin D treatment. B Effects of UPF1 and LIN00963 inhibition on SOX2, OCT4, and NANOG expression assessed using western blotting. The results are presented as the ratio of the integrated density values of SOX2, OCT4, and NANOG versus Tubulin. The graphs represent the alteration in relation to the sh-UPF1-NC + sh-LINC00963-NC group (protein of interest/Tubulin equal to 1). C Effects of UPF1 and LIN00963 inhibition on self-renewal capacity assessed using serial sphere formation assay. D Effects of UPF1 and LIN00963 inhibition on carboplatin resistance assessed using the sphere formation assay. E Effects of UPF1 and LIN00963 inhibition on carboplatin resistance assessed using the CCK8 assay. F Effects of UPF1 and LIN00963 inhibition on proliferation assessed using the CCK8 assay. G Effects of UPF1 and LIN00963 inhibition on migration and invasion assessed using the Transwell assay. H Effects of UPF1 and LIN00963 inhibition on apoptosis assessed using flow cytometry analysis. I Effects of UPF1 and LIN00963 inhibition on cell cycle progression assessed using flow cytometry analysis. Data are presented as the means ± SEM (n = 3, each group), *P < 0.05, **P < 0.01, ***P < 0.001 vs. sh-UPF1-NC + sh-LINC00963-NC group, #P < 0.05, ##P < 0.01, ###P < 0.001 vs. sh-UPF1 + sh-LINC00963-NC group, △P < 0.05, △△P < 0.01, △△△P < 0.001 vs. sh-UPF1-NC + sh-LINC00963 group. Scale bars, 50 μm.

Back to article page