Fig. 2: YTHDF1 knockdown inhibited cell growth and invasion in vitro. | Cell Death & Disease

Fig. 2: YTHDF1 knockdown inhibited cell growth and invasion in vitro.

From: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis

Fig. 2: YTHDF1 knockdown inhibited cell growth and invasion in vitro.The alternative text for this image may have been generated using AI.

A and B Western blot and RT-PCR analysis for YTHDF1 expression in MDA-MB-231 and MCF7 cells transfected with YTHDF1 -inhibiting siRNAs. C CCK8 assays on the proliferation of MDA-MB-231 and MCF7 cells after YTHDF1 knockdown. D EdU assays of MDA-MB-231 and MCF7 cells after YTHDF1 knock-down. E Colony formation assays of MDA-MB-231 and MCF7 cells after YTHDF1 knockdown. F YTHDF1 knockdown decreased the invasive potential of MDA-MB-231 and MCF7 cells. G FACS analysis on the apoptosis of MDA-MB-231 and MCF7 cells after YTHDF1 knockdown. Statistical analysis results are presented as mean ± SEM, student’s t test, *P < 0.05, ** P < 0.01, ***P < 0.001.

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