Fig. 4: miR-16-5p down-regulates YTHDF1 to inhibit breast cancer cell growth and invasion in vitro. | Cell Death & Disease

Fig. 4: miR-16-5p down-regulates YTHDF1 to inhibit breast cancer cell growth and invasion in vitro.

From: YTHDF1 upregulation mediates hypoxia-dependent breast cancer growth and metastasis through regulating PKM2 to affect glycolysis

Fig. 4: miR-16-5p down-regulates YTHDF1 to inhibit breast cancer cell growth and invasion in vitro.The alternative text for this image may have been generated using AI.

A, B Western blot analysis for YTHDF1 expression in MDA-MB-231 and MCF7 cells transfected by miR-16-5p mimics/ miR-16-5p inhibitors or co-transfection with miR-16-5p mimics and pCDNA3.1-YTHDF1-3×FLAG. (C and D) CCK8 assays on breast cancer cell growth after the combined treatment of miR-16-5p mimics/miR-16-5p inhibitors or co-transfection with miR-16-5p mimics and pCDNA3.1-YTHDF1-3×FLAG in MDA-MB-231 and MCF7 cells. E, F EdU assays of MDA-MB-231 and MCF7 cells transfected by miR-16-5p mimics/miR-16-5p inhibitors or co-transfection with miR-16-5p mimics and pCDNA3.1-YTHDF1-3×FLAG. G, H Colony formation assays of MDA-MB-231 and MCF7 cells transfected by miR-16-5p mimics/miR-16-5p inhibitors or co-transfection with miR-16-5p mimics and pCDNA3.1-YTHDF1-3×FLAG. I and J miR-16-5p mimics with/without miR-16-5p inhibitors or miR-16-5p mimics with/without pCDNA3.1-YTHDF1-3×FLAG transfection on the invasive abilities of breast cancer cells. K, L miR-16-5p mimics with/without miR-16-5p inhibitors or miR-16-5p mimics with/without pCDNA3.1-YTHDF1-3×FLAG transfection on the apoptosis levels of MDA-MB-231 and MCF7 cells by FACS. Statistical analysis results are presented as mean ± SEM, student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page