Fig. 2: miR-592 deletion impairs cortical neurogenesis and adult lineage choice.

a Double immunofluorescence staining. Pax6 staining (red immunofluorescence), p-H3 staining (green immunofluorescence), and DAPI staining (blue fluorescence). Unpaired two-tailed t test. Error bars represent S.E.M. Experiments were carried out using three biological replicates. Each sample was observed in three fields of view. b BrdU staining (red immunofluorescence) and DAPI staining (blue fluorescence). Unpaired two-tailed t test. Error bars represent S.E.M. Experiments were carried out using three biological replicates. Each sample was observed in three fields of view. c E14.5–P7 sagittal sections were immunostained for the radial glial marker SOX2 (pink), the IPCs marker TBR2 (green), and the neuronal marker MAP2 (red). Nuclei were labelled with DAPI. d E14.5–P7 RT-PCR showed that TBR2⁺ cells were dysregulated in the SVZ in miR-592^−/− mice beginning at E14.5. VZ/SVZ ventricular/subventricular zone (PAX6 and SOX2), IZ the intermediate zone (TBR2), CP cortical plate (TBR1 and CITP2), MZ marginal zone (MAP2). e–h Double immunofluorescence staining. e Tuj1 staining (red immunofluorescence), GFAP staining (green immunofluorescence), and DAPI staining (blue fluorescence). f Tuj1 staining (red immunofluorescence), Iba1 staining (green immunofluorescence), and DAPI staining (blue fluorescence). g Tuj1 staining (red immunofluorescence), Olig2 staining (green immunofluorescence), and DAPI staining (blue fluorescence). h Syn1 staining (red immunofluorescence), PSD95 staining (green immunofluorescence), and DAPI staining (blue fluorescence). All data were analysed by two-way ANOVA followed by Tukeys multiple comparison test. Error bars represent S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001. The averages of nine different fields of view were calculated for each animal (counts from three fields of three sagittal slices).