Fig. 3: Genetic deletion of miR-592 reduces motor coordination and social interaction.

a Timeline of tests for extensive behaviours including movement and coordination, social behaviours, learning and memory. b–d OFT, open field test. b Representative images showing typical examples of the exploration behaviour of WT and miR-592^−/− mice during the first 5 min of the open field test. c The time spent in the centre and d the velocity was scored for each 5 min period (unpaired two-tailed t test, n = 8 mice for each genotype, error bars represent S.E.M.). e RT rotarod test. The time on the rotating bar was recorded in the rotarod test (unpaired two-tailed t test, n = 8 mice for each genotype, error bars represent S.E.M.). f–h EPM elevated plus maze test. f Representative images showing typical examples of the exploration behaviour of WT and miR-592^−/− mice during the first 5 min of the elevated plus-maze test are provided. The time spent by WT and miR-592^−/− mice in g closed arms and h open arms during the elevated plus-maze assay was quantified (unpaired two-tailed t test, n = 8 mice for each genotype, error bars represent S.E.M.). i LDT light–dark transition test. The latency to the first entry into the light chamber in the light/dark box assay is shown (unpaired two-tailed t test, n = 8 mice for each genotype, error bars represent S.E.M.). j–m MWM Morris Water Maze. j Representative images showing typical examples of exploring the behaviour of WT and miR-592^−/− mice during the first 10 min of the water maze task are provided. k The average velocity, l time spent in the target-quadrant areas, and m frequency of entry into the target quadrants were recorded after 7 days of training session (unpaired two-tailed t test, n = 8 mice for each genotype, error bars represent S.E.M.). n Timeline of tests for social interaction behaviours. o Self-grooming. Self-grooming assessed over a 10 min period (one-way ANOVA with Tukey’s multiple comparison test, n = 8 mice for each genotype, error bars represent S.E.M.). p NOP novel object preference test. Object exploration was allowed to test the novelty preference of WT and miR-592^−/− mice (two-way ANOVA with Tukey’s multiple comparison test, n = 8 mice for each genotype, error bars represent S.E.M.) q, r Three-chambered test. Experimental mice were socialized by exposure to a stranger mouse over a 3-day training period and then placed with a novel mouse. q Sociability test session. r Social novelty preference test. The time spent in the stranger mouse zone was quantified (two-way ANOVA with Tukey’s multiple comparison test, n = 8 mice for each genotype, error bars represent S.E.M.). *p < 0.05, **p < 0.01, ***p < 0.001.