Table 1 Primers.
Gene | Sense | Antisense | Figure |
|---|---|---|---|
miR-592 (genotyping) | 5’-CCATGACAACCGACCCTTGA-3’ | 5’-AGCAACAACCTATATCCCACGG-3’ | Fig. 1e |
MeCP2 (genotyping) | 5’-CGCTCCGCCCTATCTCTGA-3’ | 5’-ACAGATCGGATAGAAGACTC-3’ | Data not shown |
MeCP2 (dual-Luciferase) | 5’-TGTTCTTCCTGGTGACTCTG-3’ | 5’-CCCTTGTCCTACTCTATGGT-3’ | Fig. 5b |
U6 (RT-PCR) | 5’-CGCTTCGGCAGCACATATAC-3’ | 5’-AATTTGCGTGTCATCCTTGC-3’ | Fig. 1b |
miR-592 (RT-PCR) | 5’-AAGGGATTCTGATGTTGGTCACAC-3’ | 5’-GCTGTCAACGATACGCTACGTAACG-3’ | Fig. 1b |
Sox2 (RT-PCR) | 5’-TCTCAAACTGTGCATAATGGAGTAA-3’ | 5’-CCCTTTTATTTTCCGTAGTTGTATT-3’ | Fig. 2d |
Map2 (RT-PCR) | 5’-CAATCTTCACATTACCACCTCCA-3’ | 5’-CTCTAAAGAACATCCGTCAC-3’ | Fig. 2d |
TBR1 (RT-PCR) | 5’-GCTTCGTCACAGTTTCGATGG-3’ | 5’-CCGTTGGTAATGACCGGGTG-3’ | Fig. 2d |
TBR2 (RT-PCR) | 5’-GCAATAAGATGTACGTTCACCCA-3’ | 5’-GCAGAGACTGCAACACTATCAT-3’ | Fig. 2d |
CTIP2 (RT-PCR) | 5’-CCCGACCCTGATCTACTCAC-3’ | 5’-GGAGGTGGACTGCTCTTGT-3’ | Fig. 2d |
PAX6 (RT-PCR) | 5’-TACCAGTGTCTACCAGCCAAT-3’ | 5’-TGCACGAGTATGAGGAGGTCT-3’ | Fig. 2d |
MeCP2 (RT-PCR) | 5’-TTCTATTCTGGGCTTTTGATTTGT-3’ | 5’-CCCTTGTCCTACTCTATGGTTATCA-3’ | |
Pten (RT-PCR) | 5’-TGGATTCGACTTAGACTTGACCT-3’ | 5’-GCGGTGTCATAATGTCTCTCAG-3’ | Fig. 4g |
Lrrc7 (RT-PCR) | 5’-CAAGCTCTACGGAAACTAAGCA-3’ | 5’-ACACCGTTTTTACTGATGTCGAG-3’ | Fig. 4g |
FOXP1 (RT-PCR) | 5’-CACCTCAGGTTATCACTCCTCA-3’ | 5’-AGCTGCAACTGTTCCTGTTGT-3’ | Fig. 4g |
GAPDH (RT-PCR) | 5’-GCTGTCAACGATACGCTACGTAACG-3’ | 5’-TGAAGGGGTCGTTGATCG-3’ | Fig. 4g |
Grm8 (RT-PCR) | 5’-TCCCTTCCCTCTCCAACCTAACATG-3’ | 5’-CCACGCTCTTCCAATCCTCTTTCC-3’ | Fig. S1e |
BDNF (RT-PCR) | 5’-TCATACTTCGGTTGCATGAAGG-3’ | 5’-AGACCTCTCGAACCTGCCC-3’ | Fig. S4h |
