Fig. 7: Site-specific regulatory role of Ehhadh K572cr on the transcriptome in vitro.
From: Acox2 is a regulator of lysine crotonylation that mediates hepatic metabolic homeostasis in mice
A Hierarchical clustering and Heatmap showing the profiles of differentially expressed genes (DEGs) in 293T cells expressing Ehhadh K572R when compared with Ehhadh WT. B Gene Ontology enrichment analysis revealed that “DNA topoisomerase type I (single strand cut. ATP-independent) activity” was the most significantly enriched term in the K572R vs WT group. C, D mRNA levels of TOP3B were further confirmed by cDNA PCR (C) and quantitative real-time PCR (D). *p < 0.05; ***p < 0.001 (one-way ANOVA test, n = 4). E Western blot showed protein levels of TOP3B was downregulated whereas expression of FH and HK2 was not affected in cells overexpression K572R when compared with WT and other mutants. Representative image from three independent experiments was displayed.
