Fig. 5: TRIM46 interacted with PHLPP2 and promoted PHLPP2 ubiquitination in LUAD cells. | Cell Death & Disease

Fig. 5: TRIM46 interacted with PHLPP2 and promoted PHLPP2 ubiquitination in LUAD cells.

From: TRIM46 activates AKT/HK2 signaling by modifying PHLPP2 ubiquitylation to promote glycolysis and chemoresistance of lung cancer cells

Fig. 5

A Co-IP assay of TRIM46 and PHLPP2 in H358 cells. B Immunofluorescence assay of TRIM46 and PHLPP2 in H358 cells (scale bar: 20 μm). C qPCR and immunoblot analysis of PHLPP2 expression in both H358 cells and H1299 cells with TRIM46 silence. D Analysis of PHLPP2 expression in A549 cells transfected with WT-TRIM46, RING-mutant TRIM46, or vector. E IP analysis of the ubiquitination of PHLPP2 in A549 cells transfected with WT-TRIM46, RING-mutant TRIM46, or vector. F Immunohistochemical staining of TRIM46, PHLPP2, and p-AKT in cohort 1 (scale bar: 100 μm). G, H Statistical analysis of LUAD tissues under different staining conditions in cohort 1. Results were presented as the mean ± standard error (n = 3). Independent experiments were repeated three times.

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