Fig. 2: Knockdown of hsa_circRNA_0088036 inhibits the proliferation, migration, and invasion of BCa cells.
From: Hsa_circRNA_0088036 acts as a ceRNA to promote bladder cancer progression by sponging miR-140-3p
A Verification of hsa_circRNA_0088036 knockdown by qRT-PCR analysis. B T24 and UMUC3 cells were transfected with hsa_circRNA_0088036-shRNA. Cell growth was measured with a cell proliferation assay. C, D T24 cells (C) and UMUC3 cells (D) were transfected with hsa_circRNA_0088036-shRNA. Cell migration was measured with the wound healing assay. E, F T24 cells (E) and UMUC3 cells (F) were transfected with hsa_circRNA_0088036-shRNA. 100 μL matrigel was added into upper chambers. Transwell invasion assays were performed. The invading cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. G SUSD1 expression in 72 paired human BCa and adjacent normal bladder tissues. H Correlation analysis of SUSD1 mRNA and hsa_circRNA_0088036 level was performed with the Pearson’s correlation coefficient. I The qRT-PCR analysis was performed to detect the expression of SUSD1 mRNA after overexpression of hsa_circRNA_0088036 in T24 cells and knockdown of hsa_circRNA_0088036 in UMUC3 cells. J Western blot analysis was performed to detect the expression of SUSD1 after overexpression of hsa_circRNA_0088036 in T24 cells and knockdown of hsa_circRNA_0088036 in UMUC3 cells.
