Fig. 2: Effects of CDC42EP3 depletion on cellular phenotypes in vitro.
From: CDC42EP3 promotes glioma progression via regulation of CCND1
A Results of CCK8 assay indicated a significant inhibition on cell proliferation after depleting CDC42EP3 in SHG-44 and U251 cells. B Higher level of cell apoptosis following downregulation of CDC42EP3 than controls was detected by FACS (shCtrl vs. shCDC42EP3) in both cell lines. C Comparison of percentages of cells in three cell cycle phases (shCtrl vs. shCDC42EP3) in both experimental cell lines. D Representative images and differences on cell migration rate detected by wound-healing assay (shCtrl vs. shCDC42EP3) in both cell lines. E Representative images (×200) and the comparison on cell migration ability resulted from Transwell assay on the experimental cells. The left panel lists results for SHG-44 cells and the right panel is for U251 cells. Data were presented by mean with SD (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001.
