Fig. 4: The differential factors secreted by edited AZIN1 compared with WT group.
From: A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
A Proteome profiler human angiogenesis array to detect the differential factors in HCT 116 cells and B co-culture of HCT 116 and HUVEC. Mean expression values of differential factors after densitometric quantification are shown. The grayscale value of IL-8 is circulated by a red rectangular box. C ELISA Quantitative determination of IL-8 from conditioned medium of each group. Data are presented as means ± SD. n = 3. Data were analyzed with unpaired Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. D IL-8 mRNA expression was shown in colon cancer compared to para-cancer tissue. E Volcano plot revealed differentially expressed genes between colon cancer and para-cancer tissue. F Enrichment of genes with the hallmark pathways by GSEA analysis. G GSEA analysis was performed to identify the correlation of angiogenesis-related genes and IL-8. H Correlation between IL-8 and angiogenesis-related gene signatures. Spearman rank p value and correlation coefficient are shown.
