Fig. 5: AMTB exhibits antitumoral function through repressing the TGFβ signaling pathway. | Cell Death & Disease

Fig. 5: AMTB exhibits antitumoral function through repressing the TGFβ signaling pathway.

From: AMTB, a TRPM8 antagonist, suppresses growth and metastasis of osteosarcoma through repressing the TGFβ signaling pathway

Fig. 5

A, B RT-qPCR was conducted to detect the transcriptional level of TGFβ and relevant targets of TGFβ signaling after treatment with various concentrations of AMTB. C Western blot assay on P-Smad2 and P-Smad3 indicated that AMTB can inhibit the phosphorylation of Smad2 and Smad3 in a dose-dependent manner. Western Blot is representative of three independent western blot with different lysates. D By adding TGFβ (5 ng/ml) to AMTB-treated osteosarcoma cells, the phosphorylation level of Smad2 and Smad3 was rescued to basal level. Western blot is representative of three independent western blot with different lysates. E, F Annexin V/PI staining revealed a variation in apoptosis rate in 143B cells and U2OS cells after different treatment, and their proliferation ability was assessed by the colony formation assay. The data was compared by one-way ANOVA with Tukey’s multiple comparison test and shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.

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